The Sphingolipid in Agaricus bisporus

Agrobacterium tumefaciens mediated transformation of Agaricus bisporus

Agrobacterium tumefaciens is known to transfer parts of its tumor-inducing plasmid to filamentous fungi including Agaricus bisporus. We have used this transformation system to transform germinating basidiospores of A. bisporus. Analysis of hygromycin B-resistant clones showed that the T-DNA integrated at random sites and in multiple copies into the host genome. Furthermore we have been able to ...

Virus-Enriched Fraction from Agaricus bisporus

Partially purified virus preparations from sporophores of Agaricus bisporus affected with LaFrance disease had up to a 15-fold-higher RNA-dependent RNA polymerase activity than did comparable preparations from healthy sporophores. Enzyme activity was dependent upon the presence of Mg2+ and the four nucleoside triphosphates and was insensitive to actinomycin D, a-amanitin, and rifampin. The 3H-l...

Germination of Lecanicillium fungicola in the mycosphere of Agaricus bisporus.

Dry bubble disease is a major problem in the commercial cultivation of the white button mushroom Agaricus bisporus and is caused by the ascomycete Lecanicillium fungicola. In the casing layer, germination of L. fungicola spores is inhibited by the microflora, a phenomenon known as fungistasis. Fungistasis is annulled when the casing is colonized by A. bisporus hyphae. We demonstrated that addit...

The cel4 gene of Agaricus bisporus encodes a beta-mannanase.

Mannases have industrial uses in food and pulp industries, and their regulation may influence development of the mushrooms of commercially important basidiomycetes. We expressed an Agaricus bisporus cel4 cDNA, which encodes a mannanase, in Saccharomyces cerevisiae and Pichia pastoris. CEL4 had no detectable activity on cellulose or xylan. This gene is the first isolated from this economically i...

The cel4 Gene of Agaricus bisporus Encodes a b-Mannanase